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one step sybr primescript rtpcr kit  (TaKaRa)


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    Structured Review

    TaKaRa one step sybr primescript rtpcr kit
    One Step Sybr Primescript Rtpcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 10927 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sybr+primescript+rtpcr+kit/PrimeScript+RT-PCR+Kit/pm41299730-56-6-12
    Average 98 stars, based on 10927 article reviews
    one step sybr primescript rtpcr kit - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Effects of Sucrose Feeding on the Quality of Royal Jelly Produced by Honeybee Apis mellifera L.
    Article Snippet: Insects 2023, 14, x FOR PEER REVIEW 4 of 12 from 1 μg total RNA by reverse transcription using a Transcript All-in-One First-Strand cDNA Synthesis SuperMix (TransGen Biotech, Beijing, China) following instructions. .. Reverse transcription quantitative PCR (qRT-PCR) was carried out on a ABI 7500 Real Time PCR System (Applied Biosystems, Waltham, MA, USA) with an SYBR PrimeScript RTPCR kit (Takara, Beijing, China). ..

    Article Title: Par6/SOX2 interact to modulate stemness maintenance in glioma by regulating the EGFR/PI3K/AKT signaling cascade.
    Article Snippet: Total RNA was extracted from various glioma cell lines using TRIzol reagent (Invitrogen) according to the manufacturer’s instructions. .. The extracted RNA was reverse-transcribed and amplified using the SYBR PrimeScript RTPCR Kit (TaKaRa Bio, Kusatsu, Japan) with a CFX96 Real-Time PCR Detection System (Bio-Rad, Hercules, CA). ..

    Article Title: Differentiation and Maturation Effect of All-Trans Retinoic Acid on Cultured Fetal RPE and Stem Cell-Derived RPE Cells for Cell-Based Therapy.
    Article Snippet: .. Total RNA was extracted using TRIzol reagent (TaKaRa) and cDNA was synthesized with a reverse transcription kit (TaKaRa) according to the manufacturer’s instructions. qRT-PCR was carried out using the SYBR PrimeScript RTPCR kit (TaKaRa). ..

    Article Title: Lithium Chloride Exerts Anti-Inflammatory and Neuroprotective Effects by Inhibiting Microglial Activation in LPS-Induced Retinal Injury.
    Article Snippet: Total RNA from microglia or retinas were extracted using TRIzol reagent (Thermo Fisher Scientific) according to the manufacturer’s protocol, followed by treatment with DNAse I to remove DNA contaminants. .. The RNA was then reverse transcribed into cDNA by using a SYBR PrimeScript RTPCR Kit (Takara Biomedical Technology, Beijing, China). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Effects of Sucrose Feeding on the Quality of Royal Jelly Produced by Honeybee Apis mellifera L.
    Article Snippet: Insects 2023, 14, x FOR PEER REVIEW 4 of 12 from 1 μg total RNA by reverse transcription using a Transcript All-in-One First-Strand cDNA Synthesis SuperMix (TransGen Biotech, Beijing, China) following instructions. .. Reverse transcription quantitative PCR (qRT-PCR) was carried out on a ABI 7500 Real Time PCR System (Applied Biosystems, Waltham, MA, USA) with an SYBR PrimeScript RTPCR kit (Takara, Beijing, China). ..

    Article Title: Par6/SOX2 interact to modulate stemness maintenance in glioma by regulating the EGFR/PI3K/AKT signaling cascade.
    Article Snippet: Total RNA was extracted from various glioma cell lines using TRIzol reagent (Invitrogen) according to the manufacturer’s instructions. .. The extracted RNA was reverse-transcribed and amplified using the SYBR PrimeScript RTPCR Kit (TaKaRa Bio, Kusatsu, Japan) with a CFX96 Real-Time PCR Detection System (Bio-Rad, Hercules, CA). ..

    Article Title: All-trans-retinoic acid suppresses rat embryo hindlimb bud mesenchymal chondrogenesis by modulating HoxD9 expression.
    Article Snippet: Densitometric analysis was performed using Quantity One software version 4.5.2 (Bio-Rad, Hercules, CA, USA). .. For real-time PCR, reactions were performed using the SYBR PrimeScript RTPCR kit (Takara Bio, Dalian, China) according to the manufacturer’s instructions in an ABI 7500 system (Applied Biosystems, CA, USA). ..

    Article Title: A Comprehensive Analysis of Potential Gastric Cancer Prognostic Biomarker ITGBL1 Associated With Immune Infiltration and Epithelial–mesenchymal Transition
    Article Snippet: Subsequently, cDNA was synthesized by reverse transcription with PrimeScript RT kits (Takara Biotechnology Co., Japan) following the manufacturer’s protocol. .. Subsequently, generated cDNA was ampli ed with an SYBR PrimeScript RTPCR kit (Takara Biotechnology Co.) on an ABI 7900 Real-Time PCR system. ..

    Article Title: Black phosphorus nanosheets encapsulated microneedle for multifunctional therapy for androgenic alopecia.
    Article Snippet: Total RNA was extracted with the Trizol reagent (Invitrogen, Thermo Fisher Scientific, Inc., Waltham, MA, USA), and reverse transcription was performed using the PrimeScript RT-PCR kit (Takara, Dalian, China) according to the manufacturer’s protocol. .. RT-PCR was performed with the SYBR PrimeScript RTPCR kit (Takara), Power SYBR Green PCR Master Mix, and a real-time PCR Cycler (Roche LightCycle 480II, BIOTECON Diagnostics). .. Table 1 lists the sequences of the specific primers (Sangon Biotech, Shanghai, China) used in RT-PCR.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Effects of Sucrose Feeding on the Quality of Royal Jelly Produced by Honeybee Apis mellifera L.
    Article Snippet: Insects 2023, 14, x FOR PEER REVIEW 4 of 12 from 1 μg total RNA by reverse transcription using a Transcript All-in-One First-Strand cDNA Synthesis SuperMix (TransGen Biotech, Beijing, China) following instructions. .. Reverse transcription quantitative PCR (qRT-PCR) was carried out on a ABI 7500 Real Time PCR System (Applied Biosystems, Waltham, MA, USA) with an SYBR PrimeScript RTPCR kit (Takara, Beijing, China). ..

    Article Title: Endoplasmic Reticulum Stress and the Lysosomal Pathway Play Crucial Roles in the Progression of βB2-Crystallin Mutation-Induced Congenital Cataracts in Mice.
    Article Snippet: .. Realtime PCRs were performed using the SYBR PrimeScript RTPCR kit (Takara, Siga, Japan) in 20 μL reactions with an ABI Prism 7000 sequence detection system (Applied Biosystems, Foster City, CA, USA) according to the Minimum Information for Publication of Quantitative RT PCR Experiments (MIQE) guidelines.28 All the primers were designed to cross introns. ..

    Article Title: Par6/SOX2 interact to modulate stemness maintenance in glioma by regulating the EGFR/PI3K/AKT signaling cascade.
    Article Snippet: Total RNA was extracted from various glioma cell lines using TRIzol reagent (Invitrogen) according to the manufacturer’s instructions. .. The extracted RNA was reverse-transcribed and amplified using the SYBR PrimeScript RTPCR Kit (TaKaRa Bio, Kusatsu, Japan) with a CFX96 Real-Time PCR Detection System (Bio-Rad, Hercules, CA). ..

    Article Title: Differentiation and Maturation Effect of All-Trans Retinoic Acid on Cultured Fetal RPE and Stem Cell-Derived RPE Cells for Cell-Based Therapy.
    Article Snippet: .. Total RNA was extracted using TRIzol reagent (TaKaRa) and cDNA was synthesized with a reverse transcription kit (TaKaRa) according to the manufacturer’s instructions. qRT-PCR was carried out using the SYBR PrimeScript RTPCR kit (TaKaRa). ..

    Article Title: All-trans-retinoic acid suppresses rat embryo hindlimb bud mesenchymal chondrogenesis by modulating HoxD9 expression.
    Article Snippet: Densitometric analysis was performed using Quantity One software version 4.5.2 (Bio-Rad, Hercules, CA, USA). .. For real-time PCR, reactions were performed using the SYBR PrimeScript RTPCR kit (Takara Bio, Dalian, China) according to the manufacturer’s instructions in an ABI 7500 system (Applied Biosystems, CA, USA). ..

    Article Title: A Comprehensive Analysis of Potential Gastric Cancer Prognostic Biomarker ITGBL1 Associated With Immune Infiltration and Epithelial–mesenchymal Transition
    Article Snippet: Subsequently, cDNA was synthesized by reverse transcription with PrimeScript RT kits (Takara Biotechnology Co., Japan) following the manufacturer’s protocol. .. Subsequently, generated cDNA was ampli ed with an SYBR PrimeScript RTPCR kit (Takara Biotechnology Co.) on an ABI 7900 Real-Time PCR system. ..

    Article Title: Lithium Chloride Exerts Anti-Inflammatory and Neuroprotective Effects by Inhibiting Microglial Activation in LPS-Induced Retinal Injury.
    Article Snippet: Total RNA from microglia or retinas were extracted using TRIzol reagent (Thermo Fisher Scientific) according to the manufacturer’s protocol, followed by treatment with DNAse I to remove DNA contaminants. .. The RNA was then reverse transcribed into cDNA by using a SYBR PrimeScript RTPCR Kit (Takara Biomedical Technology, Beijing, China). ..

    Article Title: Black phosphorus nanosheets encapsulated microneedle for multifunctional therapy for androgenic alopecia.
    Article Snippet: Total RNA was extracted with the Trizol reagent (Invitrogen, Thermo Fisher Scientific, Inc., Waltham, MA, USA), and reverse transcription was performed using the PrimeScript RT-PCR kit (Takara, Dalian, China) according to the manufacturer’s protocol. .. RT-PCR was performed with the SYBR PrimeScript RTPCR kit (Takara), Power SYBR Green PCR Master Mix, and a real-time PCR Cycler (Roche LightCycle 480II, BIOTECON Diagnostics). .. Table 1 lists the sequences of the specific primers (Sangon Biotech, Shanghai, China) used in RT-PCR.

    Sequencing:

    Article Title: Endoplasmic Reticulum Stress and the Lysosomal Pathway Play Crucial Roles in the Progression of βB2-Crystallin Mutation-Induced Congenital Cataracts in Mice.
    Article Snippet: .. Realtime PCRs were performed using the SYBR PrimeScript RTPCR kit (Takara, Siga, Japan) in 20 μL reactions with an ABI Prism 7000 sequence detection system (Applied Biosystems, Foster City, CA, USA) according to the Minimum Information for Publication of Quantitative RT PCR Experiments (MIQE) guidelines.28 All the primers were designed to cross introns. ..

    Quantitative RT-PCR:

    Article Title: Endoplasmic Reticulum Stress and the Lysosomal Pathway Play Crucial Roles in the Progression of βB2-Crystallin Mutation-Induced Congenital Cataracts in Mice.
    Article Snippet: .. Realtime PCRs were performed using the SYBR PrimeScript RTPCR kit (Takara, Siga, Japan) in 20 μL reactions with an ABI Prism 7000 sequence detection system (Applied Biosystems, Foster City, CA, USA) according to the Minimum Information for Publication of Quantitative RT PCR Experiments (MIQE) guidelines.28 All the primers were designed to cross introns. ..

    Article Title: Differentiation and Maturation Effect of All-Trans Retinoic Acid on Cultured Fetal RPE and Stem Cell-Derived RPE Cells for Cell-Based Therapy.
    Article Snippet: .. Total RNA was extracted using TRIzol reagent (TaKaRa) and cDNA was synthesized with a reverse transcription kit (TaKaRa) according to the manufacturer’s instructions. qRT-PCR was carried out using the SYBR PrimeScript RTPCR kit (TaKaRa). ..

    Amplification:

    Article Title: Par6/SOX2 interact to modulate stemness maintenance in glioma by regulating the EGFR/PI3K/AKT signaling cascade.
    Article Snippet: Total RNA was extracted from various glioma cell lines using TRIzol reagent (Invitrogen) according to the manufacturer’s instructions. .. The extracted RNA was reverse-transcribed and amplified using the SYBR PrimeScript RTPCR Kit (TaKaRa Bio, Kusatsu, Japan) with a CFX96 Real-Time PCR Detection System (Bio-Rad, Hercules, CA). ..

    Synthesized:

    Article Title: Differentiation and Maturation Effect of All-Trans Retinoic Acid on Cultured Fetal RPE and Stem Cell-Derived RPE Cells for Cell-Based Therapy.
    Article Snippet: .. Total RNA was extracted using TRIzol reagent (TaKaRa) and cDNA was synthesized with a reverse transcription kit (TaKaRa) according to the manufacturer’s instructions. qRT-PCR was carried out using the SYBR PrimeScript RTPCR kit (TaKaRa). ..

    Generated:

    Article Title: A Comprehensive Analysis of Potential Gastric Cancer Prognostic Biomarker ITGBL1 Associated With Immune Infiltration and Epithelial–mesenchymal Transition
    Article Snippet: Subsequently, cDNA was synthesized by reverse transcription with PrimeScript RT kits (Takara Biotechnology Co., Japan) following the manufacturer’s protocol. .. Subsequently, generated cDNA was ampli ed with an SYBR PrimeScript RTPCR kit (Takara Biotechnology Co.) on an ABI 7900 Real-Time PCR system. ..

    SYBR Green Assay:

    Article Title: Black phosphorus nanosheets encapsulated microneedle for multifunctional therapy for androgenic alopecia.
    Article Snippet: Total RNA was extracted with the Trizol reagent (Invitrogen, Thermo Fisher Scientific, Inc., Waltham, MA, USA), and reverse transcription was performed using the PrimeScript RT-PCR kit (Takara, Dalian, China) according to the manufacturer’s protocol. .. RT-PCR was performed with the SYBR PrimeScript RTPCR kit (Takara), Power SYBR Green PCR Master Mix, and a real-time PCR Cycler (Roche LightCycle 480II, BIOTECON Diagnostics). .. Table 1 lists the sequences of the specific primers (Sangon Biotech, Shanghai, China) used in RT-PCR.

    Polymerase Chain Reaction:

    Article Title: Black phosphorus nanosheets encapsulated microneedle for multifunctional therapy for androgenic alopecia.
    Article Snippet: Total RNA was extracted with the Trizol reagent (Invitrogen, Thermo Fisher Scientific, Inc., Waltham, MA, USA), and reverse transcription was performed using the PrimeScript RT-PCR kit (Takara, Dalian, China) according to the manufacturer’s protocol. .. RT-PCR was performed with the SYBR PrimeScript RTPCR kit (Takara), Power SYBR Green PCR Master Mix, and a real-time PCR Cycler (Roche LightCycle 480II, BIOTECON Diagnostics). .. Table 1 lists the sequences of the specific primers (Sangon Biotech, Shanghai, China) used in RT-PCR.



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